Saturday, July 18, 2020
Value and invest in yourself
Value and invest in yourself If you are into financial investment, youre probably very familiar with Mr. Warren Buffet (1951â"present). He is the most successful investor in the world. His investment strategies are legendary and many people seek to learn after him. Even more respectable, he pledged to give away 99% of his wealth (more than $30 billions at the time of the pledge in 2006) to non-profit foundations, mostly to Bill Gates Foundation. Mr. Buffet often travels to universities to give speeches to educate and motivate students. Here is one of his speeches to teach us the value of our body, to invest in ourselves, in education for a great future. Imagine that a Genie offers you any car in the world. The catch is that it is the only car you will ever own. What would you do?You would read the manual ten times, change the oil twice as often as required, and you would take fastidious care so that that car remained the car of your dreams forever.Think about what this tells you about your body.You get only o ne mind and one bodythe same ones you will have at 20, 40, 60, etc.Take care of them and maximize their potential. It will be too late to take care of your body and mind (and car) later on. You can maintain them, but it is hard or impossible to undo big mistakes or negligence later on. You do not want to end up with a wreck on your hands.Your main asset in life is yourself.Treat yourself as a valuable asset. I often explain to students that I would be willing to pay today for a percentage of the future earnings of good students.If you value yourself, and invest in yourself, you will be worth a great deal through out your lifetime, both to yourself and to your community.Author: Warren Buffet
Thursday, May 21, 2020
Insight From Theory And History - 1638 Words
Essay 1 Insights from Theory and History When referring to the subject of International Political Economy the main focus of study in this field looks at analyzing and finding reasons for the problems that arise or are affected from the interaction of international political decision, international economics, international trade, as well as different social systems and societal groups. Over the course and development of these systems prominent figures of economic theory and government interaction have emerged throughout the years. Two of the most influential theorist that have come to the fore front have been John Maynard Keyes, with his theory of Keynesian economics and Milton Friedman with his idea of monetarism. While both economists have had major influence on modern day economic policies, both theorist have contradicting ideas, this paper will aim to take a look at both Keynes and Friedmanââ¬â¢s theories respectively and look at the application of these theories in the real world. John Maynard Keynes theory of economics rose to prominence following the Wall Street stock market crash of 1929. This crash resulted in a major economic plummet, astonishing rise in unemployment, business closures, and a decline in tax revenues. Due to the occurrence of these events the American government turned to borrowing money from other countries. In addition to borrowing money in an effort to slow the recession, the government used strategies of classical economic theory proposed by AdamShow MoreRelatedThe Fundamental Knowledge Of Knowledge1637 Words à |à 7 Pagesknowledge issue arising from this question is whether knowledge is consistent throughout time. Can we progress through applying knowledge generated decades ago? To determine whether knowledge generated from areas such as history and science can predict the future, it is necessary to know whether the knowledge acquired through these areas of knowing is consistent, irrespective of changes in time and culture, amongst other factors. Through considering this question, we are gaining insight into whether we shouldRead MoreKohlers Theories: A Comparative Analysis to Freuds Approach and Contemporary Relevance795 Words à |à 3 Pagesï » ¿ Kohler: comparison to Freud and Contemporary Relevance. Kohler was the father of insight psychology and seemingly one of the precursors of behaviorism. He concluded that learning is a trial-and error process that depends on rewards and punishments. Kohler learned this form his studies with chimps and other animals such s dogs and cats, where, he showed that through insight behavior animals learned how to achieve their goals and which types of conduct to avoid (Hothersall , 1995). His first experimentRead MoreEssay on The History and Heritage of Society1671 Words à |à 7 PagesTo understand heritage, an assessment of history and heritage needs to be examined. It can be suggested that history and heritage conceive of and use the past in similar ways. History accepts the existence of episodes from the past in much the same way as geography assumes the existence of places hat can be described, however imperfectly, as really existing even if not directly experienced by the narrator, on the basis of whatever record is available and selected for use (Tunbridge and Ashworth 1996)Read More The Unwritten History of Cave Paintings Essay1613 Words à |à 7 Pages Religious stories and scientific theories have tried for thousands of years to accurately date the formation of man. However, in the 1860s, a new art was discovered that changed the view of human creation forever. This new insight was cave painting. Cave painting is a form of prehistoric art that dates b ack hundreds of thousands of years. Analysts are unable to specify the reasoning behind these paintings of objects on cave walls, but many theories have been formulated to surmise the rationalRead MoreRace, Hegemony, and Cultural Participation1699 Words à |à 7 PagesAfrican American women through American history. The paper connects sociological theories as a way to explain and contemplate hegemony, equality, and social relations. The paper analyzes two quotations from real and fictional characters that aptly circumscribe their experience in relation to White American hegemony. The paper concludes that theories such and Hegemony Theory, Functionalist Theory, and Feminist Nationalist Theory offer precise and practical insight to the experience and oppression of AfricanRead MoreTheory Analysis of the Experiments of Kohler743 Words à |à 3 PagesKohler was the father of insight psychology and seemingly one of the precursors of behaviorism. He concluded that learning is a trial-an d error process that depends on rewards and punishments. Kohler learned this form his studies with chimps and other animals such s dogs and cats, where, he showed that through insight behavior animals learned how to achieve their goals and which types of conduct to avoid (Hothersall , 1995). His first experiment was to place food at the other side of a barrier. TheRead MoreAn Insight Of. Erikson Theory On Aging. Anthony Isamade.968 Words à |à 4 Pages An Insight of Erikson Theory on Aging Anthony Isamade Oluwatosin Abegunde Nursing Care Concepts Aging Jean Eaton January 27, 2017 Abstract A perception of old age and the crisis associated with it. Erikson theory illuminates these various issues associated with old age through Ego Integrity vs Despair, and generates ideas that the future generations now employ to understand Aging. An Insight of Erikson Theory on Aging Aging is a concept that impacts our society. WithRead MorePeter Drucker891 Words à |à 4 Pagesmanagement philosophy was, and is still considered to this present day as revolutionary, described as a man ââ¬ËWho could see around cornersââ¬â¢ Richard Straub briefly discusses how history was a prominent and integral element to all Drucker wrote. The article emphases Druckerââ¬â¢s ambition to shape and influence management theory and practice, he once quoted ââ¬ËThe best way to predict the future is to create itââ¬â¢. The article outlines not only how Drucker has created management strategies relevant for todaysRead MoreCritical Analysis Of Nursing Concepts1213 Words à |à 5 Pagespast and present nursing concepts, and how these theories have evolved. The initial paper by J. Fawcett (1984) describes the fundamental concepts of nursing including person, environment, health and nursing, (Fawcett, 1984 p.84) The author further describes ideas that are discussed and compa red by various nursing scholars and how they are related to one another. In the second paper by Newman, Smith, et al, (2008) they explore the current theories and how more advanced these are, and where the studiesRead MoreBlack Folk Analysis On The Double Consciousness Theory Essay908 Words à |à 4 Pages Re-examining W.E.B Du Boisââ¬â¢ Souls of Black Folk analysis on the double consciousness theory INTRODUCTION: In 1903 the brilliant historican and sociologist W.E.B Du Boisââ¬â¢ introduced his theory known as the Double-Conciousness in Souls of Black Folk Bois provides his readers with insight on the treatment of people of color, and supports this notion by giving personal examples of his experiences. He writes with the hope that many will understand the diffculty of having to be obtain two personalities
Wednesday, May 6, 2020
The Social Construction of Whiteness and Race in America
The Social Construction of Whiteness and Race in America A comment was made in a blog post early on in the year about whiteness in American that bugged me. Itââ¬â¢s a topic that came up a few times throughout the semester in and outside of class. Granted, this topic is based on a single blog post but a collection of comment and statements that were made on specific blogs and during class sessions. This topic I fin extremely important mainly because I felt as though there was some confusion around the topic being white. Understandably if youââ¬â¢re white in America I think itââ¬â¢s easy to forget exactly how privileged you are. Nonetheless, it forced me to want to talk about white privilege in America, explain the meaning of ââ¬Å"paradox of privilegeâ⬠,â⬠¦show more contentâ⬠¦Tim wise uses pronouns such as ââ¬Å"weâ⬠and ââ¬Å"ourâ⬠many times in speeches. He uses these pronouns to describe people in general. He is describing essentially every person . He is giving a message and making a statement out to people all over the world when using the many pronouns that he does, by fighting for a society in which those privileges will no longer exist and we will be able to stand on our own two feet without the artificial crutch of racial advantage to prop us up. ââ¬Å"We need to commit to fighting for racial equity and challenging injustice at every turn, not only because it harms others, but because it diminishes us as well (even as it pays dividends), and because it squanders the promise of fairness and equity to which we claim to adhere as Americans.â⬠(Page 18 Paragraphs 18) From this quote it is clear that Wise is not just singling one group of people out he is talking to everyone (mainly Americans). ââ¬Å"It is only when you step back, stop looking at the wires one by one, microscopically, and take a macroscopic view of the whole cage, that you can see why the bird does not go anywhere; and then you will see it in a moment. It will require no great subtlety of mental powers. It is perfectly obvious that the bird is surrounded by a network of systematically related barriers, no one of which would be the least hindrance to its flight, but which, by their relations to each other, are as confining as the solid wallsShow MoreRelatedRace As A Social Construction1679 Words à |à 7 PagesAlisha Sparks Dr. Stanley POLS 24 November 2015 Race as a Social Construction When we talk about race, what are we really talking about? The issue of race is a complex issue, with socially ambiguous undertones that have plagued our society for decades. Race has been a marker and maker of stereotypes. Race has been used as a justification for injustice. Whether slavery, Japanese internment, or social and economic exclusion, race has given an avenue for those in power to exclude ones deemed ââ¬Ëotherââ¬â¢Read MoreThe White Scourge, By Neil Foley1104 Words à |à 5 Pagesand Poor Whites in Texas Cotton Culture In The White Scourge, Neil Foley gives detailed facts about the construction and reconstruction of whiteness and the connection of this whiteness to power, mainly on cotton culture in central Texas. Foley s book analyzes ââ¬Å"whitenessâ⬠through detailed analysis of race, class, and gender. What was most intriguing about this book is its comparison of whiteness on various racial groups and classes, for and how each struggled in comparison to the other in order toRead MoreDiversity Is An Important Element Of The United States1430 Words à |à 6 Pagesof the United States of America, but also has been a topic debated over for many years. The arrival of immigrants in the United States in the 19th and early 20th century created many problems with how to deal with new cultures and naturalization of citizens of different races. These immigrants faced much adversity in the process of gaining citizenship due to racial qualifications for naturalization and fitting into separate race categories. Since white was the superior race in the United States, immigrantsRead MoreThe Wages Of Whiteness By David Roediger912 Words à |à 4 Pagesrelationship between race, capitalism, and property and how it perpetuates the notion of whiteness through the exploitation of ââ¬Å"othersâ⬠. Property is a relationship of a person and an object; slaves were considered as objects. Race is constructed from white workersââ¬â¢ ideology of whiteness and labor wage. Racism has been long constructed through the production of race and its relations to property, and we can see it through the notion of capitalism and the idea of whiteness. In the Wages of Whiteness (an editionRead MoreEssay on Whiteness in Danzy Sennaââ¬â¢s Novel, Caucasia 821 Words à |à 4 Pagesconstructed. It is a heartfelt story about the conflicts of an interracial family in an extremely racialised America during the 1970s. This novel treads on the border between whiteness and blackness through the standpoint of Birdie Lee. Birdie is able to manipulate her identity and fit into different spaces as required by her mother, Sandy Lee. Throughout the novel, the reader is shown how race is socially constructed, and how essentially everyone ââ¬Å"pretendsâ⬠in their life in order to fit certain situationsRead MoreWhiteness and Citizenship971 Words à |à 4 PagesCaptain Ahabââ¬â¢s eulogy of whiteness shows that the word ââ¬Å"whiteâ⬠implies more than a chromatic description. ââ¬Å"Whiteâ⬠is an untenable perfection that has haunted the American psyche since colonial times. The idea of ââ¬Å"white spiritual superiorityâ⬠can only be enforce by a terrorist politico-legal system, based on brutalizing the non-whites and creating a national fantasy. A national fantasy defined by Lauren Berlant as the means ââ¬Å"to designate how national culture becomes local through the images, narrativesRead MoreThe Naturalization Process During The Late 19th And Early 20th Century1486 Words à |à 6 Pagesnotion of superiority that was associated with being ââ¬Å"whiteâ⬠. When immigrants soon realized the value and importance of ââ¬Å"whitenessâ⬠in Americaââ¬â¢s society, they quickly applied for citizenship. In response to their movement, the government created racial and non-racial requirements that made it very difficult for people from African and Asian descent to justify their ââ¬Å"whitenessâ⬠. The naturalization process during this time was a very intricate and complex procedure. American courts struggled provingRead MoreWhiteness Of A Different Color1363 Words à |à 6 PagesMatthew Frye Jacobsonââ¬â¢s Whiteness of a Different Color offers innovative insight into the concept of ââ¬Å"raceâ⬠and the evolution of ââ¬Å"whitenessâ⬠throughout American history. Jacobson focuses his analysis on the instability of racial identification over time and how race has been created and perceived throughout different stages of history. He states in his introduction that ââ¬Å"one of the tasks before the historian is to discover which racial categories are useful to whom at a given moment, and whyâ⬠(pRead MoreThe History Of White People By Nell Irvin952 Words à |à 4 Pagesliteral adaptation of the title. The book is about how the ââ¬Å"white raceâ⬠came about; it discusses how certain ethnicities, and races came to be considered white, or Caucasian. I believe the book probably contains a lot more history, however she skipped over most of that and touched on the main points. The concept of race is a human construction. Painter called it an idea. The main point of the video is the concept of ââ¬Å"whitenessâ⬠, and how it has evolved over time. She begins by discussing the ideaRead MoreWhite Privilege : Unpacking The Invisible Backpack891 Words à |à 4 PagesJohnson (privilege, oppression, and difference) and Peggy McIntosh (White Privilege: Unpacking the Invisible Backpack) discuss their views on the impact that privileging specific social groups has on society. The concept of whiteness in America is often overlooked or unaffiliated with discussions concerning racial dilemmas. Whiteness and white privilege perpetuates racism due to its normalization in society which in turn serves as an agent of how other non-white people are treated. White privilege has
J. Pierpont Morgan and Andrew Carnegie Free Essays
Viviana Kucharski AMH 2020 Sep 20 2012 J. Pierpont Morgan Andrew Carnegie It is difficult to image two men born in such a different social condition have so much in common. Two men that opened his way to the success based in hard work and somehow shared that success with others less fortunate. We will write a custom essay sample on J. Pierpont Morgan and Andrew Carnegie or any similar topic only for you Order Now J P Morgan and Andrew Carnegie were two powerful men; disciplined, intelligent, and hard workers. President Roosevelt described J P Morgan as a sincere and trustful man. He developed a social and professional confidence, and his corpulent body and deep black eyes were intimidating. He was born as a natural leader. In the other hand Andrew Carnegie was compassionate, ethic, humble, a truth optimistic. His mother taught him the value of hard work. ââ¬Å"He was devoted to his mother and deeply lover her. â⬠(www. biography. com, minute10ââ¬â¢55ââ¬â¢). He believed in qualities of all men. Andrew Carnegie was born in Scotland in 1835 in the womb of a working class family. Since school wasnââ¬â¢t mandatory his formal education didnââ¬â¢t start until age of 8, however his father and uncles led his early education. In the other side of the world, in Connecticut, America ââ¬Å"J P Morgan was born into a prosperous mercantile-banking family in 1837â⬠. (American Eras. Vol. 8. ) He was raised in Hartford, Boston, and London. His formal education began in New England schools and continued in Switzerland and Germany. After a year in University his father placed in one of his affiliate firms. Instead, once Andrew arrived to America at age of 13, his schoolââ¬â¢s days were over. Andrewââ¬â¢s first job was a bobbin boy and then in the Oââ¬â¢Reilly Telegraph Company. ââ¬Å"Andrew saw this job as a first step on his road toward success. â⬠(Machen Rau, pg 36. After years or learning and observing, Andrew invested in the Adams Express Company and in the Woodruff Sleeping Car Company and it turned out to be a successful investment that brought him lots of money. Unlike Carnegie, Morgan first job was on the board of a financial firm. Morgan possessed a unique skill at transforming strugg ling business and management to make them highly profitable, and this includes the remorganization of the railroad dramatically affected by the depression of 1893, and the Government of U. S. A. , which he saved twice, first in the Gold treasury Crisis and later in the depression of 1907. Meanwhile, in 1863 Andrew realized that wood structures could be replaced by iron. He formed the Keystone Bridge Company and after years of doubts he finally invested in the Carnegie Steel Company, which Morgan would buy years after to form in 1901 the U. S. Steel Company. In two decades J P Morgan managed the formation of different companies including the well-known General Electric and Carnegie turned his life from being a bobbin boy to being the richest man in the world. Andrew Carnegie and J P Morgan left a tremendous legacy focused in education, scientific research, and foundations. Carnegieââ¬â¢s legacy also includes the improvement in steel quality and its production to make it more affordable. J P Morgan also left a legacy consistent in financial corporations that still working these days touching the life of thousand of people. Andrew Carnegie and J P Morgan were two powerful men that made a fantastic input in the life of millions of people helping to transform America from an agrarian nation to an industrial and economic leader of the world. Sources: â⬠¢ ââ¬Å"Andrew Carnegie: Prince of Steelâ⬠2012. Biography. com Authors: Rick Davis, Jack Perkins, Jaffe Productions; Hearts Entertainment Publisher: New York, New York. Bio. 2008, 1995 http://www. biography. com/people/andrew-carnegie-9238756 â⬠¢ ââ¬Å"J. Pierpont Morgan: emperor of Wall Streetâ⬠2012 Biography. com Authors: Jack Perkins; Jaffe Productions; Actuality Productions; Hearts Entertainment Publisher: New York: A ; E Home Video: Distributed in the U. S. by New Video Group, 1996 http://www. biography. com/people/jhon-pierpont-morgan-9414735 â⬠¢ ââ¬Å"John Pierpont Morgan. â⬠American Eras. Vol 8. Detroit: Gale, 1997. Gale Biography In Context. Gale Document Number: Gale|K2438000252 â⬠¢ Dana, Meachen. Captain of Industry. Minneapolis: Compass Point Books, 1971. How to cite J. Pierpont Morgan and Andrew Carnegie, Papers
Sunday, April 26, 2020
Prophet Essays (276 words) - Christian Eschatology, Purgatory
Prophet In the prophet it does not tell you how to live, but it tells you how life works. I do not agree with everything that is said, but as a whole I like the message the book sends. I have taken my five favorite chapters, and broken them down for this report. Eating and Drinking- I think he is saying that we do not have to become vegetarians, but we must never take more than we need. I think this applies well to average killing of meat, but when he says we should be sorrowful and apologize to an apple that we bite is where I do not agree. Giving- This is one of the few chapters I totally agreed with. My favorite quote came from this chapter, "You give but little when you give of your possessions. It is when you give of yourself that you truly give". And then they speak of how the average man does not know how the poor person feels until they have been poor themselves. Crime and Punishment- He takes an interesting stance on punishment by suggesting a sort of purgatory. He says that, "And for that wrong committed must you knock and wait a while unheeded at the gate of the blessed". He also says the form of a punishment is living with the crime you did. That you will feel sorrow for what you have done. Death- He says that death is the same as life. They are one, "like the river and sea are one." This is the last chapter, because I think it is the hardest for us to understand. And I do not think that we can ever understand death, as we are living. Though, we should not be afraid of it.
Wednesday, March 18, 2020
The Difference Between a High School Diploma and a GED
The Difference Between a High School Diploma and a GED Thereââ¬â¢s more than one way to prove your knowledge. While many students spend years earning their high school diplomas, others take a battery of tests in a single day and move on to college with a General Equivalency Diploma (GED). But is a GED as good as an actual diploma? And do colleges and employers really care which one you choose? Take a look at the facts before deciding how to complete your high school education. GED Students who take the GED exam must not be enrolled in or graduated from high school and must be over the age of 16. Depending on the state where the test is taken, students may also have to meet other requirements. Requirements: The GED is awarded afterà a student passes a series of tests in five academic subjects. In order to pass each test, the student must score higher than 60% of the sample set of graduating seniors. Generally, students need to spend a considerable amount of time studying for the exams. Length of study: Students are not required to take traditional courses in order to earn their GED. The examinations take seven hours and five minutes to complete. Students may need to take preparation courses in order to get ready for the exams. However, these preparation courses are not mandatory. How employers view a GED: The majority of employers hiring for entry-level positions will consider a GED score as comparable to an actual diploma. A small number of employers will consider the GED inferior to a diploma. If a student continues school and receives a college degree, his employer will probably not even consider how he completed his high school education. How colleges view a GED: Most community colleges admit students who have received a GED. Individual universities have their own policies. Many will accept students with a GED, but some will not view the credential the same way as a diploma, especially if the school requires specialized courses of study for admittance. In many cases, a traditional diploma will be viewed as superior. High School Diploma Laws vary from state to state, but most schools will permit students to work on completing their high school diploma at a traditional public school for one to threeà years after they turn eighteen. Special community schools and other programs often provide older students the opportunity to complete their graduation requirements. School diplomas do not generally have minimum age requirements. Requirements: In order to receive a diploma, students must complete coursework as dictated by their school district. Curriculum varies from district to district. Length of study: Students generally take four years to complete their high school diploma. How employers view a diploma: A high school diploma will allow students to meet the education requirements for many entry-level positions. Generally, employees with diplomas will earn significantly more than those without. Students who wish to advance in their careers may need to attend college for additional training. How colleges view a diploma: Most students admitted to four-year colleges have earned a high school diploma. However, a diploma does not guarantee acceptance. Factors such as grade point average (GPA), coursework, and extracurricular activities also play a role in admissions decisions.
Monday, March 2, 2020
Gram Stain Procedure in Microbiology
Gram Stain Procedure in Microbiology The Gram stain is a differential method of staining used to assign bacteria to one of two groups (gram-positive and gram-negative) based on the properties of their cell walls. It is also known as Gram staining or Grams method. The procedure is named for the person who developed the technique, Danish bacteriologist Hans Christian Gram. How the Gram Stain Works The procedure is based on the reaction between peptidoglycan in the cell walls of some bacteria. The Gram stain involves staining bacteria, fixing the color with a mordant, decolorizing the cells, and applying a counterstain. The primary stain (crystal violet) binds to peptidoglycan, coloring cells purple. Both gram-positive and gram-negative cells have peptidoglycan in their cell walls, so initially, all bacteria stain violet.Grams iodine (iodine and potassium iodide) is applied as a mordant or fixative. Gram-positive cells form a crystal violet-iodine complex.Alcohol or acetone is used to decolorize the cells. Gram-negative bacteria have much less peptidoglycan in their cell walls, so this step essentially renders them colorless, while only some of the color is removed from gram-positive cells, which have more peptidoglycan (60-90% of the cell wall). The thick cell wall of gram-positive cells is dehydrated by the decolorizing step, causing them to shrink and trapping the stain-iodine complex inside.After the decolorizing step, a counterstain is applied (usually safranin, but sometimes fuchsine) to color the bacteria pink. Both gram-positive and gram-negative bacteria pick up the pink stain, but it is no t visible over the darker purple of the gram-positive bacteria. If the staining procedure is performed correctly, gram-positive bacteria will be purple, while gram-negative bacteria will be pink. Purpose of the Gram Staining Technique The results of the Gram stain are viewed using light microscopy. Because the bacteria are colored, not only is their Gram stain group identified, but their shape, size, and clumping pattern may be observed. This makes the Gram stain a valuable diagnostic tool for a medical clinic or lab. While the stain may not definitely identify bacteria, often knowing whether they are gram-positive or gram-negative is sufficient for prescribing an effective antibiotic. Limitations of the Technique Some bacteria may be gram-variable or gram-indeterminate. However, even this information may be useful in narrowing down bacterial identity. The technique is most reliable when cultures are less than 24 hours old. While it can be used on broth cultures, its best to centrifuge them first. The primary limitation of the technique is that it yields erroneous results if mistakes are made in the technique. Practice and skill are needed to produce a reliable result. Also, an infectious agent may not be bacterial. Eukaryotic pathogens stain gram-negative. However, most eukaryotic cells except fungi (including yeast) fail to stick to the slide during the process. Gram Staining Procedure Materials Crystal violet (primary stain)Grams iodine (mordant, to fix crystal violet in the cell wall)Ethanol or Acetone (decolorizer)Safranin (secondary stain or counterstain)Water in a squirt bottle or dropper bottleMicroscope slidesCompound microscope Note its better to use distilled water than tap water, as pH differences in water sources may affect results. Steps Place a small drop of bacterial sample on a slide. Heat fix the bacteria to the slide by passing it through the flame of a Bunsen burner three times. Applying too much heat or for too long can melt the bacteriaà cell walls, distorting their shape and leading to an inaccurate result. If too little heat is applied, the bacteria will wash off the slide during staining.Use a dropper to apply the primary stain (crystal violet) to the slide and allow it to sit for 1 minute. Gently rinse the slide with water no longer than 5 seconds to remove excess stain. Rinsing too long can remove too much color, while not rinsing long enough may allow too much stain to remain on gram-negative cells.Use a dropper to apply Grams iodine to the slide to fix the crystal violet to the cell wall. Let it sit for 1 minute.Rinse the slide with alcohol or acetone about 3 seconds, followed immediately with a gentle rinse using water. The gram-negative cells will lose color, while the gram-positive cells will rema in violet or blue. However, if the decolorizer is left on too long, all cells will lose color! Apply the secondary stain, safranin, and allow it to sit for 1 minute. Gently rinse with water no longer than 5 seconds. The gram-negative cells should be stained red or pink, while the gram-positive cells will still appear purple or blue.View the slide using a compound microscope. A magnification of 500x to 1000x may be needed to distinguish cell shape and arrangement. Examples of Gram-Positive and Gram-Negative Pathogens Not all bacteria identified by the Gram stain are associated with diseases, but a few important examples include: Gram-positive coccià (round)à -Staphylococcus aureusGram-negative coccià -à Neisseria meningitidisGram-positive bacilli (rods)à -à Bacillus anthracisGram-negative bacillià -à Escherichia coli
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